Department of Reproduction and Clinic of Farm Animals

Department of Reproduction and Clinic of Farm Animals

Address
Pl. Grunwaldzki 49
50-366 Wrocław

Contact (Secretary)
Małgorzata Wiewióra, sam. referent
phone 71 320 5302
email This email address is being protected from spambots. You need JavaScript enabled to view it.

Head of Department
prof. dr hab. Wojciech Niżański
phone 71 320 5315
email This email address is being protected from spambots. You need JavaScript enabled to view it.

Zarodek kota w stadium balstocysty
zapłodnienie metodą ICSI
b IMG 3796
badanie kliniczne 1
chirurgia 1
chirurgia 2
MG 3799
IMG 5670
IMG 20190411 115638
aObraz7
bObraz8
cObraz10
dObraz12
eObraz13
fObraz14
g
hpracownia biologii molekularnej
ipracownia zapłodnienia in vitro 2
j pracownia zapłodnienia in vitro 3
k warsztaty 7
l warsztaty

Rozrod team2018

Staff
prof. dr hab. Jan Twardoń, phone 71 320 5306 email This email address is being protected from spambots. You need JavaScript enabled to view it.
prof. dr hab. Roland Kozdrowski phone 71 320 5307 email This email address is being protected from spambots. You need JavaScript enabled to view it.
dr Wiesław Bielas, adiunkt, phone 71 320 5310 email This email address is being protected from spambots. You need JavaScript enabled to view it.
dr Grzegorz Dejneka, adiunkt, phone 71 320 5317 email This email address is being protected from spambots. You need JavaScript enabled to view it.
dr hab. Michał Dzięcioł, prof. nadzw., phone 71 320 5313 email This email address is being protected from spambots. You need JavaScript enabled to view it.
dr Krzysztof Janeczko, adiunkt, phone 71 320 5313 email This email address is being protected from spambots. You need JavaScript enabled to view it.
dr hab. Małgorzata Ochota, adiunkt, phone 71 320 5300 email This email address is being protected from spambots. You need JavaScript enabled to view it.
dr hab. Agnieszka Partyka, adiunkt phone 71 320 5300 email This email address is being protected from spambots. You need JavaScript enabled to view it.
dr Sylwia Prochowska, adiunkt phone 71 320 5303 email This email address is being protected from spambots. You need JavaScript enabled to view it.
Katarzyna Tomasik, st. technik phone 71 320 5308 email This email address is being protected from spambots. You need JavaScript enabled to view it.
mgr Jadwiga Godlejewska–Piątek, st. technik, phone 71 320 1052 email This email address is being protected from spambots. You need JavaScript enabled to view it.
mgr inż. Edyta Kowalska, st. technik phone71 320 5308 email This email address is being protected from spambots. You need JavaScript enabled to view it.
mgr Barbara Smalec phone 71 320 5314 email This email address is being protected from spambots. You need JavaScript enabled to view it.
mgr inż. Marta Bilska phone 71 320 5303 email This email address is being protected from spambots. You need JavaScript enabled to view it.
Mariusz Kochel, laborant phone 71 320 5305
Piotr Michalski, laborant phone 71 320 5305
Małgorzata Wiewióra, sam. referent phone 71 320 5302 email This email address is being protected from spambots. You need JavaScript enabled to view it.

PhD students
lek. wet. Agnieszka Bartoszewicz, doktorantka email This email address is being protected from spambots. You need JavaScript enabled to view it.
lek. wet. Bartłomiej M. Jaśkowski, doktorant, email This email address is being protected from spambots. You need JavaScript enabled to view it.
lek. wet. Natalia Krasowska, doktorantka, email This email address is being protected from spambots. You need JavaScript enabled to view it.
lek. wet. Barbara Pieczewska, doktorantka phone 71 320 53 11 email This email address is being protected from spambots. You need JavaScript enabled to view it.
lek. wet. Olga Rodak, doktorantka phone 71 320 53 11 email This email address is being protected from spambots. You need JavaScript enabled to view it.
lek. wet. Katarzyna Wojtysiak, doktorantka phone 71 320 53 09 email This email address is being protected from spambots. You need JavaScript enabled to view it.
lek. wet. Martyna Woszczyło, doktorantka email This email address is being protected from spambots. You need JavaScript enabled to view it.
lek. wet. Anna Górka
lek. wet. Zuzanna Ligocka

Professors emeritus:
prof. dr hab. Andrzej Dubiel,
prof. dr hab. Stanisław Raułuszkiewicz

Research

Research: Diagnosis and therapy of reproductive disorders in animals using conventional methods and techniques of assisted reproduction

The team conducts constant research on the physiology and pathology of reproduction of domestic animals and wildlife. They concern the processes of reproduction control, diagnosis and treatment of diseases of the reproductive system, increasing and optimizing reproductive potential and productivity of animals. The experiments are extended on the issues of endocrinology, cytology, microbiology of the reproductive system and new imaging techniques. The studies are conducted on the practical use of assisted reproductive technology in the collection and storage of male and female gametes, the in vitro maturation and fertilization and also embryo transfer. The research is also carried out on somatic cloning (Nuclear Transfer) of endangered species. Especially interesting direction of the research work is to develop reliable methods for males reproductive potential evaluation with the use of the modern diagnostic techniques including flow cytometry and computer assisted sperm analysis of motility and morphology.
Studies are extended on the analysis of the expression of selected genes using RT PCR. Clinical analysis is also extended on MRI and ultrasound color Doppler. In the search for optimal and reliable methodology to evaluate the levels of sex hormones simultaneously ELFA technique and chemiluminescence is applied. The studies are carried out on various species, including cattle, horses, dogs, pigs, domestic and wild felines, sheep, goats, rabbits, hares, deer, bison and poultry.

Research projects:

Mechanisms of semiochemical communication in canines in the context of sexual behavior: the research with the use of the domestic dog (Canis familiaris) as a model species. Project of National Centre of Science (NCN) 2015/17/B/NZ8/02411. 2016–2018. Leader prof. dr hab. Wojciech Niżański

Improvement of effectiveness of wild cats genetic resources protection program by establishment of cell banks and implementation of in vitro embryo production to the practice. Project of National Center of Research and Development (NCBiR) NR PBS3/B8/16/2015. 2014 – 2018. Leader prof. dr hab. Wojciech Niżański

Development of prototypes of medical devices based on materials derived from antlerogenic stem cells. Project NCBiR UODDEM- 1- 351/001. 2014-2016.
Task 7: Research on the use of medical devices: assessment of the impact of homogenate from antlerogenic stem cells on the semen quality and results of insemination in animals; application research confirming the positive impact of prototypes based on homogenate from antlerogenic stem cells on semen quality and results of artificial insemination. Leader of the task prof. dr hab. Wojciech Niżański
Task 8: Research on the use of medical devices: assessment of the impact of homogenate from antlerogenic stem cells on the semen quality and results of insemination in animals; application research confirming the positive impact of prototypes - nutrition supplements based on homogenate from antlerogenic stem cells on semen quality and results of artificial insemination. Leader of the task prof. dr hab. Wojciech Niżański
Task 11: Research on the use of medical devices: the possibility of applying the product from antlerogenic stem cells in treating / supporting infertility treatment of cows and mares due to impaired function of the endometrium. Leader of the task prof. dr hab. Wojciech Niżański
Task 12: Research on the use of medical devices: the possibility of applying the product from antlerogenic stem cells in treating / supporting treatment of clinical and subclinical inflammatory of bovine mastitis; application research confirming the positive impact and usefulness of prototypes for the treatment / supporting the treatment of diseases of the mammary gland of cows. Leader of the task dr hab. Michał Dzięcioł

Immunoassays to measure and detect progesterone in cow's milk. Project NCBiR PBS2/B8/15/2014. 2014-2017. Key investigator prof. dr hab. Jan Twardoń

Prospective study in the tolerance of a combination of Milbemycine Oxime and Praziquantel in Breeding, Lactating Bitches and in newborn puppies. Project ordered by Virbac Company. 2015- 2016. Leader prof. dr hab. Wojciech Niżański

Quality, intensity of apoptosis and usefulness for in vitro techniques of the cat semen collected from the urethra and epididymis. Project NCN N308 576240. 2011-2014. Leader prof. dr hab. Wojciech Niżański

Comparison study on the effectiveness of osaterone acetate and deslorelin acetate on prostate gland in dogs. Project ordered by przez Virbac Company. 2012- 2014. Leader prof. dr hab. Wojciech Niżański

Assessment of performances of the test Ovulation test for the semi-quantitive dosage of progesterone in bitches. Projekt ordered by BVT Company. 2013-2014. Leader prof. dr hab. Wojciech Niżański

Oxidative status and lipid peroxidation in the dog semen used in assisted reproductive techniques. Project NCN N308 573339. 2010-2013. Leader prof. dr hab. Wojciech Niżański

Computer assisted analysis of the sperm motility and morphology of fresh and cryopreserved dog semen Project NCN N308 576240. 2011-2013. Leader prof. dr hab. Wojciech Niżański

Genetic and biochemical determinants of impaired fertility of stallions. Project NCN, N N0818/B/P01/40. Leader prof. dr hab. Kazimierz Kosiniak-Kamysz, Uniwersytet Rolniczy w Krakowie. Key investigator prof. dr hab. Wojciech Niżański

Research on the effects of low temperatures on developmental competence of vitrified embryos, as one of the strategies preservation of genetic resources of the family Felidae.Project NCN UMO- 2011/03/B/NZ9/03412. 2012 – 2015. Leader dr Małgorzata Ochota

Correlation of biochemical parameters and vital functions of dairy cows on the need to create a biocybernetic model of cow organism. Project NCN 49/14-W/2011/G. 2011-2014. Leader prof. dr hab. Jan Twardoń

Evaluation of mares endometrium showing subclinical endometritis based on a microbiological, cytological, histological and molecular study. Project NCN UMO-2011/01/B/NZ5/04173. 2011-2014. Leader dr hab. Roland Kozdrowski

The effect of antioxidants addition during chicken semen cryopreservation on sperm in vitro and in vivo characteristics and genes related with apoptosis expression. Project NCN N N311 5300 40. 2011-2014. Leader dr Agnieszka Partyka

The search of the basis of hereditary syndrome of inverted sex in dogs with karyotype 78, XX. Project NCN UMO- 2012/05/B/NZ9/00907. 2013-2016. Leader Prof. Dr hab. M. Świtoński, Department of Genetic and Animal Breeding, Uniwersytet Przyrodniczy w Poznaniu. Key investigator prof. dr hab. Wojciech Niżański

Modification of the cholesterol content in the chicken sperm cell membranes in order to improve the viability and motility of sperm in cryopreserved semen. Project MNiSW Iuventus Plus 0401/IP1/2011/ 71. 2012-2014. Leader dr Agnieszka Partyka

Publications:

• Ochota M., Niżański W. Time of early cleavage affects the developmental potential of feline preimplantation embryos in vitro. Theriogenology 2017, 89: 26-31.
• Prochowska S., Niżański W., Partyka A. Low levels of apoptotic-like changes in fresh and cryopreserved feline spermatozoa collected from the urethra and epididymis. Theriogenology 2017, 88: 43-49.
• Niżański W., Partyka A., Prochowska S. Evaluation of spermatozoal function-useful tools or just science. Reproduction in Domestic Animals 2016, 51(1): 37-45.
• Prochowska S, Niżański W, Partyka A. Comparative analysis of in vitro characteristics of fresh and frozen-thawed urethral and epididymal spermatozoa from cats (Felis domesticus). Theriogenology 2016, 86(8): 2063-72.
• Orsztynowicz M., Pawlak P., Podstawski Z., Niżański W., Partyka A., Gotowiecka M., Kosiniak-Kamysz K. and Lechniak D. Mitochondrial DNA copy number in spermatozoa of fertile stallions. Reproduction in Domestic Animals 2016, 51: 378–385.
• Ochota, M., Wojtasik, B., Niżański, W. Total cell number and its allocation to trophectoderm and inner cell mass in in vitro obtained cats' blastocysts. Reproduction in Domestic Animals 2016, 51(3): 339-345.
• Mańkowska M, Stachowiak M, Graczyk A, Ciazynska P, Gogulski M, Niżański W, Świtoński M. Sequence analysis of three canine adipokine genes revealed an association between TNF polymorphisms and obesity in Labrador dogs. Animal Genetics 2016, 47(2): 245-249.
• Ochota M., Pasieka A., Niżański W. Superoxide dismutase and taurine supplementation improves in vitro blastocyst yield from poor-quality feline oocytes. Theriogenology 2016, 85(5): 922-7.
• Szczerbal I., Nowacka-Woszuk J., Dzimira S., Atamaniuk W., Niżański W., Świtoński M. A rare case of testicular disorder of sex development in a dog (78, XX; SRY-Negative) with male external genitalia and detection of copy number variation in the region upstream of the SOX9 gene. Sexual Development 2016, 10(2): 74-78.
• Partyka A., Bonarska-Kujawa D., Sporniak M., Strojecki M., Niżański W. Modification of membrane cholesterol and its impact on frozen-thawed chicken sperm characteristics. Zygote 2016, 24(5): 714-23.
• Sikora M., Król J., Nowak M., Stefaniak T., Aubertsson G., Kozdrowski R. The usefulness of uterine lavage and acute phase protein levels as a diagnostic tool for subclinical endometritis in Icelandic mares. Acta Veterinaria Scandinavica 2016, 58(1): 50.
• Marcinkowska-Swojak M., Szczerbal I., Pausch H., Nowacka-Woszuk J., Flisikowski K., Dzimira S., Niżański W., Payan-Carreira R., Fries R., Kozlowski P., Świtoński M. Copy number variation in the region harboring SOX9 gene in dogs with testicular/ovotesticular disorder of sex development (78,XX; SRY-negative). Scientific Reports 2015, 5:14696.
• Prochowska S., Niżański W., Ochota M., Partyka A. Characteristics of urethral and epididymal semen collected from domestic cats. A retrospective study of 214 cases. Theriogenology 2015, 84(9): 1565-71.
• Partyka A., Niżański W., Bratkowska M., Maślikowski P. Effects of N-acetyl-L-cysteine and catalase on the viability and motility of chicken sperm during liquid storage. Reproductive Biology 2015, 15(2): 126-9.
• Kozdrowski R., Sikora M., Buczkowska J., Nowak M., Raś A., Dzięcioł M. Effects of cycle stage and sampling procedure on interpretation of endometrial cytology in mares. Animal Reproduction Science 2015, 154 :56-62.
• Król J., Twardoń J., Mrowiec J., Podkowik M., Dejneka G., Dębski B., Nowicki T., Zalewski W. Short communication: Streptococcus canis is able to establish a persistent udder infection in a dairy herd. Journal of Dairy Science 2015, 98(10): 7090-6.
• Prochowska S., Niżański W., Ochota M., Partyka A. Effect of dilution rate on feline urethral sperm motility, viability, and DNA integrity. Theriogenology 2014, 82: 1273-1280.
• Nowacka-Woszuk J., Szczerbal I., Salamon S., Kociucka B., Jackowiak H., Prozorowska E., Slaska B., Rozanska D., Orzelski M., Ochota M., Dzimira S., Lipiec M., Niżański W., Switonski M. Testicular disorder of sex development in four cats with a male karyotype (38,XY; SRY-positive). Animal Reproduction Science 2014, 151(1-2): 42-8.
• Dzięcioł M., Stańczyk E., Noszczyk-Nowak A., Michlik K., Kozdrowski R., Niżański W., Pasławska U., Mrowiec J., Twardoń J. The influence of Sildenafil citrate on uterine tissue perfusion and the cardiovascular system during the luteal phase of the ovarian cycle in cows. Acta Histochemica 2014, 116(2): 377-81.
• Salamon S., Nowacka-Woszuk J., Szczerbal I., Dzimira S., Nizanski W., Ochota M., Switonski M. A lack of association between polymorphisms of three positional candidate genes (CLASP2 , UBP1, and FBXL2) and canine disorder of sexual development (78,XX; SRY -negative). Sexual Development 2014, 8(4): 160-5.
• Buczkowska J., Kozdrowski R., Nowak M., Raś A., Staroniewicz Z., Siemieniuch M.J. Comparison of the biopsy and cytobrush techniques for diagnosis of subclinical endometritis in mares. Reproductive Biology and Endocrinology 2014, 12: 27.
• Dziecioł M., Nizański W., Stańczyk E., Kozdrowski R., Najder-Kozdrowska L., Twardoń J. The influence of antibiotic treatment of bitches in oestrus on their attractiveness to males during mating. Polish Journal of Veterinary Sciences 2013, 16(3): 509-16.
• Partyka A., Niżański W., Bajzert .J, Łukaszewicz E., Ochota M. The effect of cysteine and superoxide dismutase on the quality of post-thawed chicken sperm. Cryobiology 2013; 67:132-136.
• Niżański W., Partyka A., Rijsselaere T. Use of fluorescent stainings and flow cytometry for canine semenassessment. Reproduction in Domestic Animals, 2012; 47: 215-221.
• Dzięcioł M., Stańczyk E., Noszczyk-Nowak A., Niżański W., Ochota M., Kozdrowski R. Influence of bitches sex pheromones on the heart rate and other chosen parameters of blood flow in stud dogs (Canis familiaris). Research in Veterinary Science 2012, 93(3): 1241-7.
• Niżański W., Mikolajewska N., Partyka, A., Ochota M. Biotechnologies in the reproduction of felines: State of knowledge, prospects and challenges. Medycyna Weterynaryjna Veterinary Medicine-Science And Practice 2012, 68 (9), 529-533.
• Antończyk A., Niżański W., Partyka A., Ochota M., Mila H. The usefulness of Real Time Morphology software in semen assessment of teratozoospermic boars. Systems Biology in Reproductive Medicine 2012, 58: 362-368.
• Partyka A., Łukaszewicz E., Niżański W. Lipid peroxidation and antioxidant enzymes activity in avian semen. Animal Reproduction Science 2012, 134(3-4): 184-90.
• Partyka A., Łukaszewicz E., Niżański W. Effect of cryopreservation on sperm parameters, lipid peroxidation andantioxidant enzymes activity in fowl semen. Theriogenology 2012, 77(8): 1497-504.
• Mikołajewska N., Müller K., Niżański W., Jewgenow K. Vitrification of domestic cat oocytes - effect on viability and integrity of subcellular structures. Reproduction in Domestic Animals 2012, 47: 215-21.
• Rijsselaere T., Van Soom A., Maes D., Niżański W. Computer-assisted sperm analysis in dogs and cats: an updateafter 20 years. Reproduction in Domestic Animals 2012, 47: 204-7.
Partyka A, Niżański W, Ochota M. Chapter: Methods of assessment of cryopreserved semen in: Current frontiers in cryobiology ed. Igor I. Katkov, In Tech 2012, 565- 574; ISBN: 978-953-51-0191-8.

PhD and habilitations
Dr hab. Michał Dzięcioł „ Semiochemical communication in the domestic dog (Canis familiaris) in the context of sexual behavior”, 22.09.2015r.
Dr Joanna Pasikowska „ Influence of ethyl acetate, and deslorelin osaterone on the function of the reproductive system of dogs with benign prostatic hyperplasia”, 23.02.2016r., supervisor prof. dr hab. Wojciech Niżański
Dr Justyna Buczkowska „Evaluation of endometrium of infertile mares using selected diagnostic methods”, 15.12.2015r., supervisor dr hab. Roland Kozdrowski
Dr Natalia Mikołajewska „The effect of vitrification on development competence of domestic cat occytes”, 17.12.2013r., supervisor prof. dr hab. Wojciech Niżański, Prof. Katarina Jewgenow (Leibniz Institute Berlin)
Dr Ewa Stańczyk “Fetal biometry and the characteristics of flow in the arteries of the uterus and fetus during pregnancy in a dog”, 17.12.2013r., supervisor prof. dr hab. Jan Twardoń
Dr Agnieszka Antończyk „ Computer assisted analysis of motility and morphology of sperm in the fresh and cryopreserved dog semen”, 18.06.2013r., supervisor prof. dr hab. Wojciech Niżański

Laboratory equipment

1. Blood analyzer (Reflovet Plus)
2. Hematology analyzer (Mindray BC-2800 VET)
3. Computer Asisted Semen analyzer Hamilton Thorne Biosciences (IVOS)
4. Stereoscopic microscope
5. Microscope Eclipse E200 (Nikon)
6. Inverted fluorescence microscope (Olympus IX73)
7. Microplate reader Elx800 (BioTek)
8. Rigid endoscopy – for laparoscopy and vaginoskopy
9. Fiberscope Q10
10. Endoscopy for insemination
11. Ultrasound 1500 Honda (Honda)
12. Ultrasound 2000 Honda (Honda)
13. Ultrasound (Dramiński)
14. Ultrasound My Lab Gold (ESOATE)
15. Ultrasound Lab MY ONE Color Doppler (ESOATE)
16. Kit Easy Scan Ultrasound-5 positions
17. Centrifuge MPW-56 (MPW) 2x
18. Incubator Q-CELL (Poll Lab)
19. MiniVIDAS (Biomerieux) Multiparameter automatic immunoassay analyzer - analysis of hormones levels
20. The laminar chamber
21. Ultrasound-Esaote MyLab 25 Gold (Color Dppler)
22. The rigid endoscope - Ureterorenoscope URS (Storz)
23. The flow cytometer (Guava EasyCyte5)
24. Microvolume spectrophotometer (DeNovix)
25. T100 thermal cycler (Bio-Rad)
26. Thermal cycler for Real Time PCR CFX96 (Bio-Rad)
27. System for the visualization of gels Molecular Imager Gel Doc XR + (Bio-Rad)
28. Ultrafreezer (PANASONIC)
29. Freezer for programmed cryopreservation of semen, oocytes and embryos - Planner
30. Set for team ultrasound
31. Micromanipulator Narishige MM-89
32. Incubator NewBrunswick atmosphere CO2
33. Incubator Haereus

Ambulatory of Department of Reproduction
50-366 Wrocław, Pl. Grunwaldzki 49
phone 71 320 5308 – Ambulatory
phone 71 320 5318 – Horse reproduction
phone 71 320 5318 – Farm animals reproduction
open:
time Mon - Fri: 8.00-18.00
time Sat - Sun: 9.00-12.00
Open hours may change during holiday periods, holidays, etc

Pricing of laboratory services (link)
Clinic of Department of Reproduction provides veterinary services such as: semen collection and evaluation, artificial insemination, tests for pregnancy, monitoring of pregnancy, parturition help, surgical procedures of females and males (castration, sterilization, cesarean sections, etc.). The above-mentioned services relate all species of domestic animals (dog, cat, pig, cow, sheep, goat, horse, cow).
 
Specialist laboratories
phone 71 320 1052 Laboratory of Reproductive Endocrinology
phone 71 320 1052 Laboratory of Andrology
phone 71 320 1053 Laboratory of IVF
phone 71 320-1052 Laboratory of Semen Bank
phone 71 320 5300 Laboratory of RT-PCR

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