Department of Biochemistry and Molecular Biology

Department of Biochemistry and Molecular Biology

Address
ul. Norwida 31
50-275 Wrocław

Contact
phone 71 320 5209 fax 71 320 5434
email This email address is being protected from spambots. You need JavaScript enabled to view it.

Head of Department
Prof. Maciej Ugorski
phone 71 320 5209
email This email address is being protected from spambots. You need JavaScript enabled to view it.
 
1
From left: Liliana Kiczak, Anna Kołaczkowska, Jarosław Suchański, Maciej Zacharski, Maciej Ugorski, Agata Mikołajczyk, Krzysztof Grzymajło, Irena Pacura, Barbara Zasępa, Arkadiusz Miążek
 

Department's staff:
prof. dr hab. Arkadiusz Miążek phone 71 320 – 5436 email This email address is being protected from spambots. You need JavaScript enabled to view it.
dr hab. Krzysztof Grzymajło, adiunkt phone 71 320 - 5434 emaill This email address is being protected from spambots. You need JavaScript enabled to view it.
dr hab. Liliana Kiczak, adiunkt phone 71 320 – 5436 email This email address is being protected from spambots. You need JavaScript enabled to view it.
dr Rafał Kolenda, adiunkt phone 71 320 - 5434  email This email address is being protected from spambots. You need JavaScript enabled to view it.
dr hab. Anna Kołaczkowska,adiunkt phone 71 320 – 5434 email This email address is being protected from spambots. You need JavaScript enabled to view it.
dr Maciej Zacharski, adiunkt phone 71 320 - 5434 email This email address is being protected from spambots. You need JavaScript enabled to view it.
dr Izabela Sambor, specjalista phone 71 320 – 5209 email This email address is being protected from spambots. You need JavaScript enabled to view it.
dr Jarosław Suchański, adiunkt phone 71 320 - 5434 email This email address is being protected from spambots. You need JavaScript enabled to view it.
lek.wet. Agata Mikołajczyk, asystent phone 71 320 - 5434 email This email address is being protected from spambots. You need JavaScript enabled to view it.
mgr Anna Urbaniak, asystent phone 71 320-5209 email This email address is being protected from spambots. You need JavaScript enabled to view it.
mgr Barbara Suchańska, st. technikphone 71 320 – 5152 email This email address is being protected from spambots. You need JavaScript enabled to view it.
Irena Zasępa, st. technik phone 71 320 – 5152 email This email address is being protected from spambots. You need JavaScript enabled to view it.
mgr Agnieszka Jakuszak

PhD students
mgr inż. Jakub Janczara email This email address is being protected from spambots. You need JavaScript enabled to view it.
mgr Beata Klasa email This email address is being protected from spambots. You need JavaScript enabled to view it.
lek. wet. Michał Płóciennik
mgr Marcjanna Wimonć email This email address is being protected from spambots. You need JavaScript enabled to view it.

We offer bachelor/master degrees or internships in the following fields of research:

  • The role of type 1 fimbriae in Salmonella infections (contact persons: Prof. Maciej Ugorski, Dr. Krzysztof Grzymajło, Agata Mikołajczyk, D.V.M.),
  • Genotyping and phenotyping of clinical Salmonella isolates (contact persons: Prof. Maciej Ugorski, Dr. Rafał Kolenda),
  • Genomes-wide analysis of Salmonella enterica isolates with disperate type 1 fimbriae expression phenotypes (contact person: Dr. Rafał Kolenda),
  • Genome analysis of haemolytic Escherichia coli isolates with different cytotoxicity for intestinal epithelial and uroepithelial cells (contact person: Dr. Rafał Kolenda),
  • The role of ceramide galactosyltransferase (UGT8) and galactosylceramide in breast cancer progression (contact persons: Prof. Maciej Ugorski, Dr. Jarosław Suchański).

Nucleic acids analysis room

image003

General laboratory

image005

Procariotic and eucariotic cells electroporator

image007

Eucariotic cell culture room

image009

Microbiological room

biochemia2018

Cooled high speed, high capacity centrifuge

image014

Research

The role of type 1 fimbriae in Salmonella infections

Team: Maciej Ugorski, Krzysztof Grzymajło, Aleksandra Orłowska, Agata Mikołajczyk

Infection by Salmonella is initiated by the attachment and colonization of gut mucosa, which seems to be an essential step in the pathogenesis of salmonellosis. There is convincing evidence suggesting that type 1 fimbriae play an important role in bacterial survival and persistence in the host, although their exact role in adhesion, invasiveness, pathogenesis and host-specificity is still not known and controversial. Type 1 fimbriae are proteinaceous, filamentous structures present on the surface of many species of the Enterobacteriaceae, including genus Salmonella. They are composed primarily of protein subunits called FimA. However, their binding properties depend on FimH adhesin, responsible for bacteria binding to oligomannosidic structures carried by many eukaryotic membrane-bound and secreted glycoproteins [mannose-sensitive (MS) FimH adhesins]. FimH proteins of Salmonella are closely related structurally, but it has become increasingly clear that there is significant heterogeneity among type 1 fimbriae from different serovars and even from different strains of the same serovar (allelic variants). It has been suggested that minor differences in the structure of FimH are associated with differences in adhesion specificities and may determine the tropism of various Salmonella serovars to different species and tissues.

We have cloned fimH genes of S. Enteritidis, S. Typhimurium, S. Abortus-ovis, S. Choleraesuis, S. Dublin, S. Gallinarum biovar Gallinarum and S. Gallinarum biovar Pullorum. Analysis of these genes has revealed a high degree of conservation (about 97 – 99.9%) in sequenced fimH genes, with several nucleotide substitutions causing minor differences in amino acid sequences of FimH proteins. Using recombinant FimH proteins, it was shown that FimH adhesins from Salmonella enterica serovars with limited (Choleraesuis, Dublin) or restricted (Abortus-ovis) host range as well as from host unrestricted serovar Enteritidis were able to bind mannose-rich glycoproteins (RNase B, HRP and Man-BSA) with comparable affinity measured by surface plasmon resonance (Kisiela et al., 2006; Grzymajło et al., 2013). However, even though all of them represent low-adhesive variants of FimH protein, significant differences in the binding profiles of the FimH proteins of host restricted and host unrestricted serovars to enterocyte glycoproteins of sheep, pig and cattle, was observed (Grzymajło et al., 2013). When FimH adhesin from S. Enteritidis was subjected to Western blot analysis, it bound to surface membrane protein of about 130 kDa, and FimH adhesins from S. Abortus-ovis, S. Choleraesuis and S. Dublin bound to surface membrane protein of about 55 kDa. These results suggest that FimH adhesins of type 1 fimbriae are one of the factors responsible for different host-specificities of these Salmonella serovars. This hypothesis is also supported by studies on FimH adhesins from host-specific Salmonella enterica serovar Gallinarum biovars Gallinarum and Pullorum, which infect only poultry. In contrast to other analyzed Salmonella serovars, they did not bind to high-mannose oligosaccharides [mannose-resistant (MR) FimH adhesins. The loss of sugar-binding properties by biovar Gallinarum and biovar Pullorum FimH adhesins is the result of a single T78I mutation, as was demonstrated by site-directed mutagenesis of FimH proteins (Kisiela et al., 2005).

It was suggested that the expression of MS type 1 fimbriae was associated with Salmonella infections which are limited to the alimentary tract, causing gastroenteritis rather than an invasive typhoid-like disease associated with expression of MR type 1 fimbriae (Kisiela et al., 2012). Therefore, to investigate the role of the MR variant of the FimH adhesin in the pathogenesis of S. Gallinarum, the isogenic mutant elaborating type 1 fimbriae with the MS variant of the FimH adhesin from S. Enteritidis and the mutant strain with no FimH expression were constructed, and their invasiveness in 1-day-old chicks was studied. Our results demonstrated that the S. Gallinarum expressing S. Enteritidis FimH adhesin decreased systemic dissemination of S. Gallinarum and colonization of internal organs in chicks indicating the importance of the endogenous MR variant of the FimH protein in the virulence of S. Gallinarum. Elaborating from these studies, we proposed that MS variants of FimH are advantageous in gastrointestinal infections in contrast to MR FimH variants which decrease intestinal colonization and promote Salmonella systemic spreading. This hypothesis was supported by our studies on mice infected with wild-type S. Enteritidis and its fimH gene knockout strain. We observed that the loss of MS FimH adhesin correlates with the highly increased colonization of mice by these bacteria. We also showed that expression and secretion of IL-1b, IL-6, IL-10 and IL-12b was significantly higher in mice intestinal cells infected with wild-type S. Enteritidis compared to cells infected with the mutant strain. Therefore, we propose that type 1 fimbriae may play an important role in the pathogenicity of S. Enteritidis and may contribute to an intestinal inflammatory response.

Research for new bacterial virulence determinants using Omics technologies

Rafał Kolenda, Maciej Ugorski

Project in collaboration with: Peter Schierack (BTU Cottbus-Senftenberg, Germany), Gordon Dougan (Sanger Institute, UK), Josefine Bartholdson Scott (Sanger Institute, UK), Dereck Pickard (Sanger Institute, UK), Michał Burdukiewicz (Warsaw Technical University), Aamir Ali (National Institute for Biotechnology and Genetic Engineering, Pakistan), Sebastian Gunther (University of Greifswald, Germany), Torsten Semmler (Robert Koch Institute, Germany)

Advances in Omics technologies have provided us with new tools to investigate pathogenic bacteria. Adhesion and/or invasion to various epithelial cells are among the first steps during the pathogenesis of bacterial infections. Bacteria have evolved various strategies to adhere and invade host cells. To study the cell-bacteria interactions, in vitro adhesion/invasion models are often used. Currently used methods are not feasible for large scale studies of bacterial cell line infectivity. Therefore, the object was to develop an automated microscopy-based system for bacterial quantification and to screen Salmonella clinical isolates for cell line infecting phenotypes. With the use of VideoScan Technology, developed in Prof. Schierack’s group (BTU Cottbus-Senftenberg), Salmonella serovars with broad (S. Typhimurium, S. Enteritidis) and narrow (S. Gallinarum, S. Choleraesuis, S. Dublin) host ranges were screened for cell line infecting phenotypes. Bacteria were used in infection assays on three different cell lines including IPEC-J2 (porcine), Caco-2 (human) and CHIC-8E11 (avian). Adhering/infecting bacteria were counted after 1h and 4h of infection with the automated VideoScan instrument. Next, genomes of selected Salmonella isolates with the highest and lowest cell line infecting phenotypes were sequenced with next generation sequencing (NGS) at Sanger Institute in cooperation with Prof. Dougan. Currently, comparative analysis of these Salmonella genomes is being carried out. This will allow us to identify genes associated with adhesion and invasion for Salmonella clinical isolates.

The Salmonella isolates used for cell line infection assays were screened for type 1 fimbriae (T1F) expression using anti-FimH static adhesion assay (part of VideoScan). Expression of T1F was observed for more than 80% of isolates tested. Moreover, it was found that T1F are expressed in more isolates from serovars with broad host range, than in isolates from serovars with narrow host range. Next, genomes of selected Salmonella isolates with the highest and lowest T1F expression were sequenced with next generation sequencing (NGS) in cooperation with Prof. Sebastian Gunther and Dr. Torsten Semmler. Moreover, four Salmonella isolates were submitted for RNA sequencing. Subsequent analyses of Salmonella genomes and expression profiles will provide useful information about the role of genetic variability in clinical isolates of Salmonella.

The genes identified in both projects will be verified using appropriate bacterial mutants and in vitro cell line models, and in vivo animal models. The functional analysis of these genes will provide new information on the pathogenesis of Salmonella infections and will help to develop new strategies for the prevention and treatment of diseases caused by Salmonella.

The role of ceramide galactosyltransferase (UGT8) and galactosylceramide in breast cancer progression

Maciej Ugorski, Jarosław Suchański, Maciej Zacharski

Numerous studies on cellular glycoconjugates have revealed that neoplastic transformation and tumor progression are almost invariably associated with changes in the expression profiles of surface glycoproteins and glycolipids. However, there is very little information available on galactosylceramide (GalCer) expression in human tumors. GalCer is synthesized by ceramide galactosyltransferase (UGT8). Transcriptomic profiling of breast cancer tissues showed that UGT8 is one of six genes whose elevated expression correlated with a significantly increased risk of lung metastases in breast cancer patients (Landemaine et al., 2008). Based on this finding, our studies with the use of immunohistochemistry and real-time PCR on the presence of UGT8 in breast cancer tissue specimens revealed a significant increase in UGT8 expression in (1) metastatic tumors v. primary tumors, (2) tumors of malignancy grades G3 v. G2 and G3 v. G1, and (3) node-positive v. node-negative tumors (Dziegiel et al., 2010). Therefore, our data suggests that UGT8 can be a significant index of tumor aggressiveness and a potential marker for prognostic evaluation of lung metastases in breast cancer (Dziegiel et al., 2010). We have also shown, using an athymic nu/nu mice model, that the suppression of UGT8 expression in breast cancer cells has a profound effect on their tumorigenic and metastatic properties (Owczarek et al., 2013). It was found that breast cancer cells with high expression of UGT8 and GalCer form tumors much more efficiently than breast cancer cells with suppressed synthesis of GalCer after their orthotropic transplantation. Further studies with immunohistochemical staining of tumor specimens revealed that high expression of UGT8 accompanied by accumulation of GalCer in breast cancer cells is associated with a much higher proliferative index and a lower number of apoptotic cells than cells with suppressed synthesis of GalCer. Also, breast cancer cells expressing higher levels of UGT8 and synthesizing larger amounts of GalCer had a higher ability to form metastatic colonies after intracardiac inoculation into nu/nu mice. Based on these results we proposed that the presence of GalCer protects breast cancer cells from cellular stresses induced by the tumor microenvironment, and probably its cytoprotective effect is associated with increased resistance to stress-induced apoptosis. Confirming this hypothesis, it was found that expression of UGT8 and accumulation of GalCer in human breast cancer cells increased their resistance to apoptosis induced by hypoxia and free radicals as well as doxorubicin and paclitaxel. The question remains as to the exact mechanism by which GalCer mediates cytoprotective effects during stress- and drug-induced apoptosis. To answer this question we analysed the intracellular concentration of ceramide, known as a pro-apoptotic molecule, by HILIC-ESI-MS/MS. However, no differences in ceramide concentrations were found between cells synthesizing high and low amounts of GalCer. Therefore, expression of genes involved in apoptosis-related pathways was analyzed using RT2 Profiler™ PCR Array Human Apoptosis assay and Real-Time PCR using self designed primers. The level of expression of selected genes was also analysed on the level of protein using Western blotting. Gene expression profiling revealed statistically significant down-regulation of TNFRSF1B, TNFRSF9 and up-regulation of BCL2 in breast cancer cell lines with high expression of UGT8 and GalCer. These data suggest that GalCer can affect the expression of apoptosis-related genes on the level of transcription.

This work is performed in collaboration with the L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences and the Medical University of Wrocław.

Molecular bases of heart failure

Liliana Kiczak

In the framework of project “Wrovasc - Integrated Cardiovascular Centre” a porcine model of tachycardia-induced cardiomyopathy (TIC) was developed. This type of an animal model of heart failure (HF) seems to be a rational alternative for comprehensive studies of the whole spectrum of the natural history of HF, including the initial stages, which are largely under investigated (Pasławska 2011).

We investigated selected molecular elements of the parasympathetic system in the medulla oblongata (MO) in male pigs during subsequent stages of chronic TIC. Our results showed changes in the activity and levels of various elements in the parasympathetic system of MO, providing a functional link between the MO and the heart during the progression of HF. Importantly, the very early changes seen within the parasympathetic system in the MO preceded an increase in sympathetic drive. This assessment was based on high circulating catecholamines (Tomaszek 2013). Our results suggest a significant contribution of adrenals to the circulating pool of catecholamines in subjects with systolic HF (Tomaszek 2015).

Our TIC model was used to determine myocardial inflammatory factors, such as inducible NO synthase (iNOS) and oxidative stress indices during HF development. In contrast to rodents, inducible NO synthase was shown to be constitutively expressed within porcine LV. Its level decreased during the progression of systolic non-ischaemic heart failure in the pig TIC model. We found that the oxidative and inflammatory status of the failing LV myocardium did not change when compared to control animals. Thus, it can be assumed that an up-regulation of pro-inflammatory factors is not involved in HF in large mammals, and that the impact of oxidative stress may be restricted to the mitochondria in these organisms.

Another research area is myocardial remodeling in HF progression. The extracellular matrix (ECM) is supposed to play a fundamental role in this process, as well as matrix metalloproteinases (MMPs). We have demonstrated the presence of high molecular weight (HMW) complexes containing MMP9, MMP2, TIMP1 () and NGAL () in myocardium from both diseased and healthy animals. In skeletal muscles we found no HMW complexes. These HMW complexes are proteolytically inactive, but can spontaneously release active MMP9 and MMP2. This observation indicates that the HMW complexes may serve as the tissue reservoir of active MMPs, which seems to be unique for myocardial tissue (Kiczak 2013, Kiczak 2014). Investigation of sex differences in heart remodeling showed that significant LV dilatation occurred only in males, along with a strong induction of the pro-hypertrophic program. Total gelatinolytic activity in LV was higher in males than in females (irrespective of HF), and the HF progression was associated with reduced total gelatinolytic activity in the LV only in males. In females the LV dilated more slowly, without pronounced signs of eccentric hypertrophy, preserving initial diastolic function Our findings, showing sex differences in some aspects of the ECM turnover leading to a diverse myocardial response to RV pacing, highlight the need for sex-specific medicine. Furthermore, care should be taken in analyzing the results from animal HF models as some effects might be sex-specific.

Microbial multidrug resistance

Anna Kolaczkowska

Resistance to currently available drugs in microorganisms (bacteria, parasites, viruses and fungi) can be a serious threat to global public health. Infections caused by drug resistant microorganisms are difficult to treat and cause longer duration of illness. Opportunistic pathogens from the genus Candida, e.g. C. albicans and C. glabrata, are the most frequently isolated etiological factors of fungal infections. C. glabrata is mainly isolated from patients with a compromised immune system and may cause superficial but also life threatening systemic infections. In addition, C. glabrata is intrinsically less susceptible to azole drugs. It also rapidly develops resistance to antifungal agents during therapy treatment. An increased expression of genes coding membrane ATP- binding transporters (ABC transporters) extruding drugs out of the cell is the main mechanism responsible for intrinsic as well as acquired azole resistance. Apart from ABC transporter genes, other loci are involved in the development of drug resistance. To this group belong genes of the LTE (Lipid Translocating Exporters) family, loci involved in cell wall remodeling and those influencing mitochondrial status. One of the main goals is to investigate their role in drug resistance development and to understand the regulatory signals and cascades connected with this process.

Research projects:

Title, financing body, project number, period, project leader

Identification and characterization of new receptors for Salmonella enterica and their role in pathogenesis and Salmonella enterica host-specificity, NCN 2013/09/D/NZ6/02413 (SONATA). 2014-2017. Project Leader: Krzysztof Grzymajło

Research project: Advanced cancer diagnostics. Research task: Evaluation of UGT8 (UDP-galactose:ceramide galactosyltransferase), MUC1 and T antigen expression in the prognosis of various types of human malignancies. 2010-2015 No. POIG 01.01.02-2-003/08. Leader: Maciej Ugorski

Analysis of RTA gene expression in response to stress in the model organism Saccharomyces cerevisiae and in the opportunist pathogen Candida glabrata. (project NN303026537); 2009-2012; Project leader: Anna Kołaczkowska

Studies on the role of nitric oxide in the development of heart failure in pigs with tachycardia as a model of human chronic heart failure. (project MNiSW no. N N308 387837), 2009-2012. Project leader: Urszula Pasławska

Role of Type 1 fimbriae FimH adhesin as a factor of Salmonella host specificity, MNISW project IP2010011970 (IUVENTUS PLUS), 2010-2011. Project Leader: Krzysztof Grzymajło

WROVASC Integrated Cardiovascular Center, Task no. 12: Pathophysiology of heart failure progression and relationships between anabolic hormone deficiency, imbalanced autonomic nervous system and activation of inflammatory processes.   2008-2015. Innovative Economy Program (EU). Project Leader: Piotr Ponikowski, Project realized in this department by: Liliana Kiczak, Maciej Zacharski.

Study of IL-1 gene alternative transcript expression levels in cardiac muscle and other tissues of dogs with end-stage heart failure, MNiSW N N308 3037 33, 2007-2010. Project Leader: Liliana Kiczak

The role of type 1 fimbrial adhesins in the pathogenicity of Salmonella infections MNiSW 2 PO6K 023 30, 2006 – 2009. Project Leader: Maciej Ugorski.

Functional characterization of type 1 fimbrial adhesins form Salmonella Enteritidis, Salmonella Gallinarum and Salmonella Pullorum, MNiSW, 3PO6K 003 22, 2002 – 2004. Project Leader: Maciej Ugorski

Publications

2018

Sowa M., Kolenda R., Baumgart DC., Pratschke J., Papp M., Tornai T., Suchański J., Bogdanos DP., Mytilinaiou MG., Hammermann J., Laass MW., Conrad K., Schramm C., Franke A., Roggenbuck D., Schierack P. Front Immunol. 2018;9. doi:10.3389/fimmu.2018.01959. Mucosal Autoimmunity to Cell-Bound GP2 Isoforms Is a Sensitive Marker in PSC and Associated with the Clinical Phenotype. (IF: 5.51)

Kolenda R., Burdukiewicz M., Schiebel J., Rödiger S., Sauer L., Szabo I., Orłowska A., Weinreich J., Nitschke J., Böhm A., Gerber U., Roggenbuck D., Schierack P. Front Microbiol. 2018;9: 1905. doi:10.3389/fmicb.2018.01905. Adhesion of Salmonella to Pancreatic Secretory Granule Membrane Major Glycoprotein GP2 of Human and Porcine Origin Depends on FimH Sequence Variation. (IF: 4.02)

Kalicińska E., Wojtas K., Majda J., Zacharski M., Skiba J., Śliwowski J., Banasiak W., Ponikowski P., Jankowska EA. Aging Male. 2018 Sep 7:1-13. doi: 10.1080/13685538.2018.1494144. Expression of sex steroid receptors and aromatase in adipose tissue in different body regions in men with coronary artery disease with and without ischemic systolic heart failure. (IF: 2,5)

Khosrawipour, V., Mikolajczyk, A., Schubert, J., Khosrawipour, T., 2018.  Anticancer Res. 38, 3447–3452. Pressurized Intra-peritoneal Aerosol Chemotherapy (PIPAC) via Endoscopical Microcatheter System. (IF: 1,865) 

Mikolajczyk, A., Khosrawipour, V., Schubert, J., Chaudhry, H., Pigazzi, A., Khosrawipour, T., 2018. Anticancer Res. 38, 4645–4649. Particle Stability During Pressurized Intra-peritoneal Aerosol Chemotherapy (PIPAC). (IF: 1,865)

2017

Grzymajlo, K., Ugorski, M., Suchanski, J., Kedzierska, A. E., Kolenda, R., Jarzab, A., Biernatowska, A., Schierack, P. Frontiers in Cellular and Infection Microbiology. (2017). 7, 326. http://doi.org/10.3389/fcimb.2017.00326. The Novel Type 1 Fimbriae FimH Receptor Calreticulin Plays a Role in Salmonella Host Specificity. (IF:3.52)

Suchanski J., Tejchman A., Zacharski M., Piotrowska A., Grzegrzolka J., Chodaczek G., Nowinska K., Rys J., Dziegiel P., Kieda C., Ugorski M. PLoS One. 2017 Sep 22;12(9):e0184970. doi: 10.1371/journal.pone.0184970. Podoplanin increases the migration of human fibroblasts and affects the endothelial cell network formation: A possible role for cancer-associated fibroblasts in breast cancer progression. (IF: 2.804)

2016

Gomulkiewicz A., Jablonska K., Pula B., Grzegrzolka J., Borska S., Podhorska-Okolow M., Wojnar A., Rys J., Ambicka A., Ugorski M., Zabel M., Dziegiel P. Int. J. Oncol. 2016 49: 2487 – 2497. DOI: 10.3892/ijo.2016.3759. Expression of metallothionen 3 in ductal breast cancer. (IF: 3.02)

Kapelko-Slowik K., Owczarek T.B., Grzymajlo K., Urbaniak-Kujda D., Jaźwiec B., Slowik M., Kuliczkowski K., Ugorski M. Leukemia & Lymphoma 2016 Sep;57(9):2140-9 Elevated PIM2 gene expression is associated with poor survival of patients with acute myeloid leukemia. Leukemia & Lymphoma (IF: 2.891 )

Ugorski M., Dziegiel P., Suchanski J., Am. J. Cancer Res. 2016 6: 370 – 386. Podoplanin – a small glycoprotein with many faces. (IF: 4.165 )

Suchański J., Ugorski M. Postepy Hig Med Dosw 2016 70: 489 – 504.
Sulfatydy i rola biologiczna. (IF: 0.573)

Pasławska U, Kiczak L., Bania J., Pasławski R., Janiszewski A., Dzięgiel P., Zacharski M., Tomaszek A., Michlik K. Cardiovascular Pathol. 2016, 25, 3-11. doi: 10.1016/j.carpath.2015.08.003. Inducible NO synthase is constitutively expressed in porcine myocardium and its level decreases along with tachycardia induced heart failure (IF: 2.000).

Kołaczkowska A, Kołaczkowski M. Drug resistance mechanisms and their regulation in non-albicans Candida species. J Antimicrob Chemother. 2016 Jun;71(6):1438-50. doi: 10.1093/jac/dkv445. Review. (IF: 5.313)

Agata Henzel, Agnieszka Siennicka, Maciej Zacharski, Jacek Bania, Ewa A. Jankowska. Saudi J. Biomed. Res.; Vol-1, Iss-1(Mar-May, 2016):6-12. Features of type personality behaviour among young and healthy students of medicine are related to the declared level of stress but not to the level of stress measured objectively (IF: N/A)

Paleczny B, Siennicka A, Zacharski M, Jankowska EA, Ponikowska B, Ponikowski P. Clin Auton Res. 2016 Apr;26(2):107-16. doi: 10.1007/s10286-015-0338-2. Increased body fat is associated with potentiation of blood pressure response to hypoxia in healthy men: relations with insulin and leptin (IF: 1.25).

2015

Kmiecik A.M., Pula B., Suchański J., Wolbromski M., Gomulkiewicz A., Owczarek T., Kruczak A., Ambicka A., Rys J., Ugorski M., Podhorska-Okolow M., Dziegiel P. PLOS ONE 2015 10(5): e0124865. doi: 10.1371/journal.pone.0124865. Matallthionein-3 increases triple-negative breast cancer cell invasiveness via induction of metalloproteinase expression (IF: 3.234).

Tomaszek A., Kiczak L., Bania J., Pasławska U., Zacharski M., Janiszewski A., Noszczyk-Nowak A., Dzięgiel P., Kuropka P., Ponikowski P., Jankowska E. A. J. Physiol. Pharmacol. 2015, 66, 227-231. Increased gene expression of catecholamine-synthesizing enzymes in adrenal glands contributes to high circulating catecholamines in pigs with tachycardia-induced cardiomyopathy (IF: 2.720).

Kiczak L., Wałecka-Zacharska E., Bania J., Sambor I., Stefaniak T., Dzięgiel P., Zacharski M., Tomaszek A., Rybińska I., Pasławska U. Vet. Immunol. Immunopathol. 2015. 167, 91-95. doi: 10.1016/j.vetimm.2015.07.007. Anti-inflammatory properties and expression in selected organs of canine interleukin-1β splice variant 1 (IF: 1.535).

Kiczak L., Tomaszek A., Pasławska U., Bania J., Noszczyk-Nowak A., Skrzypczak P., Pasławski R., Zacharski M., Janiszewski A., Kuropka P., Ponikowski P., Jankowska E.A Biol. Sex Diff. 2015, 6:32. DOI 10.1186/s13293-015-0048-4. Sex differences in porcine left ventricular myocardial remodeling due to right ventricular pacing (IF: 4.840).

Chmielewska M., Symonowicz K., Pula B., Owczarek T., Podhorska-Okolow M., Ugorski M., Dziegiel P. Exp. Toxicol. Pathol. 2015 67: 297 – 303 doi.org/10.1016/j.etp.2015.01.006. Expression of metallothioneins I and II in kidney of doxorubicin-treated rats. (IF: 1.86)

Wyrozumska P., Meissner J., Toporkiewicz M., Szarawarska M., Kuliczkowski K., Ugorski M., Walasek M.A., Sikorski A.F. Cancer Biol. Ther. 2015 16: 66 - 76 doi.org./10.4161/15384047.2014.987009. Liposome-coated lipoplex-based carrier for antisense oligonucleotides (IF:3.072)

Tomaszek A, Kiczak L, Bania J, Pasławska U, Zacharski M, Janiszewski A, Noszczyk-Nowak A, Dzięgiel P, Kuropka P, Ponikowski P, Jankowska EA. J Physiol Pharmacol 2015, 66: 227-231. Increased gene expression of catecholamine-synthesizing enzymes in adrenal glands contributes to high circulating catecholamines in pigs with tachycardia-induced cardiomyopathy (IF: 2.72)

Kiczak L., Tomaszek A, Pasławska U, Bania J, Noszczyk-Nowak A, Skrzypczak P, Pasławski R, Zacharski M, Janiszewski A, Kuropka P, Ponikowski P, Jankowska EA. Biol Sex Diff. 2015, 6:32. DOI 10.1186/s13293-015-0048-4. Sex differences in porcine left ventricular myocardial remodeling due to right ventricular pacing (IF: 4.837)

Kiczak L, Wałecka-Zacharska E, Bania J, Sambor I, Stefaniak T, Dzięgiel P, Zacharski M, Tomaszek A, Rybińska I, Pasławska U. Vet Immunol Immunopathol. 2015. 167: 91-95. Anti-inflammatory properties and expression in selected organs of canine interleukin-1β splice variant 1 (IF: 1.535)

Kuźmińska-Bajor M., Grzymajło K., Ugorski M. Front. Microbiol. 2015 6: 276. Type 1 fimbriae are important factors limiting the dissemination and colonization of mice by Salmonella Enteritidis and contribute to the induction of intestinal inflammation during Salmonella invasion. (IF: 3.989)

2014

Kolenda R., Ugorski M., Bednarski M. Parasitol. Res. 2014 DOI 10.1007/s00436-014-3966-x. Molecular characterization of Sarcocystis species from Polish roe deer based on ssu rRNA and coxI sequence analysis (IF: 2.098).

Pula B., Wolbromski M., Owczarek T., Ambicja A., Witkiewicz W., Ugorski M., Rys J., Zabel M., Dziegiel P., Podhorska-Okolow M. Anticancer Res. 214 34: 4819 – 4828. Nogo-B receptor expression correlates negatively with malignancy grade and Ki-67 antigen expression in invasive ductal breast carcinoma (IF: 1.826).

Kiczak L, Tomaszek A, Bania J, Paslawska U, Zacharski M, Janiszewski A, Rybinska I, Dziegiel P, von Haehling S, Ardehali H, Jankowska EA, Ponikowski P. J Physiol Pharmacol. 2014 Jun;65(3):365-75. Matrix metalloproteinase 9/neutrophil gelatinase associated lipocalin/tissue inhibitor of metalloproteinasess type 1 complexes are localized within cardiomyocytes and serve as a reservoir of active metalloproteinase in porcine female myocardium (IF: 2.386)

Skrzypczak P, Zyśko D, Pasławska U, Noszczyk-Nowak A, Janiszewski A, Gajek J, Nicpoń J, Kiczak L, Bania J, Zacharski M, Tomaszek A, Jankowska EA, Ponikowski P, Witkiewicz W. Pol J Vet Sci. 2014;17(1):85-91. Effect of short-term rapid ventricular pacing followed by pacing interruption on arterial blood pressure in healthy pigs and pigs with tachycardiomyopathy. (IF: 0.604)

Paslawska U, Noszczyk-Nowak A, Paslawski R, Janiszewski A, Kiczak L, Zyśko D, Nicpon J, Jankowska EA, Szuba A, Ponikowski P. Acta Vet Scand. 2014, 56: 54. Normal electrocardiographic and echocardiographic (M-mode and two-dimensional) values in Polish Landrace pigs (IF: 1.377).

Kiczak L, Tomaszek A, Bania J, Paslawska U, Zacharski M, Janiszewski A, Rybinska I, Dziegiel P, von Haehling S, Ardehali H, Jankowska EA, Ponikowski P. J Physiol Pharmacol. 2014, 65: 365-375. Matrix metalloproteinase 9/neutrophil gelatinase associated lipocalin/tissue inhibitor of metalloproteinasess type 1 complexes are localized within cardiomyocytes and serve as a reservoir of active metalloproteinase in porcine female myocardium (IF: 2.386)

2013

Jablonska K., Pula B., Zemla A., Owczarek T., Wojnar A., Rys J., Ambicka A., Podhorska-Okolow M., Ugorski M., Dziegiel P. J. Pineal Res. 2013 54: 334 – 345. Doi:10.1111/jpl.12032. Expression of melatonin receptor MT1 in cells of human invasive ductal breast carcinoma (IF: 7.812).

Pula B., Wojnar A., Werynska B., Ambicja A., Kruczak A., Witkiewicz W., Ugorski M., Podhorska-Okolow M., Dziegiel P. Anticancer Res. 2013 33: 1447 – 1456. Impact of different tumour stroma assessment methods regarding podolanin expression on clinical outcome in patients with invasive ductal breast carcinoma (IF: 1.872).

Nowak M., Dziegiel P., Madej J., Ugorski M. Folia Histochem. Cytobiol. 2013 51:164 – 167. Ceramide galactosyltransferase (UGT8) as a molecular marker of canine mammary tumor malignancy.

Grzymajło K., Ugorski M., Kolenda R., Kędzierska A., Kuźmińska-Bajor M., Wieliczko A. Vet. Microbiol. 2013 166: 550 – 557. FimH adhesin from unrestricted Salmonella Enteritidis binds to different glycoprotein ligands expressed by enterocytes from sheep, pig and cattle than FimH adhesins from host restricted Salmonella Abortus-ovis, Salmonella Choleraesuis and Salmonella Dublin (IF: 2.726).

Pula B., Olbromski M., Wojnar A., Gomulkiewicz A., Witkiewicz W., Ugorski M., Dziegiel P., Podhorska-Okolow M. Cell Oncol. 2013 36: 469 – 483. DOI 10.1007/s13402-013-0151-7. Impact of SOX18 expression in cancer cells and vessels on the outcome of invasive ductal breast carcinoma (IF: 2.124).

Owczarek T.B., Suchański J., Pula B., Kmiecik A., Chadalski M., Jethon A., Dziegiel P., Ugorski M. PloS One 2013 8: e84191. Galactosylceramide affects tumorigenic and metastatic properties of breast cancer cells as an anti-apoptotic molecule (IF: 3.534)

Tomaszek A, Kiczak L, Bania J, Krupa P, Pasławska U, Zacharski M, Janiszewski A, Stefaniak T, Zyśko D, Ardehali H, Jankowska EA, Ponikowski P. Auton Neurosci. 2013 Oct;177(2):253-9. doi:10.1016/j.autneu.2013.05.010. Changes in parasympathetic system in medulla oblongata in male pigs in the course of tachycardia-induced cardiomyopathy (IF: 1.372)

Kiczak L, Tomaszek A, Bania J, Paslawska U, Zacharski M, Noszczyk-Nowak A, Janiszewski A, Skrzypczak P, Ardehali H, Jankowska EA, Ponikowski P. Biomed Res Int. 2013;2013:283856. doi: 10.1155/2013/283856. Expression and complex formation of MMP9, MMP2, NGAL, and TIMP1 in porcine myocardium but not in skeletal muscles in male pigs with tachycardia-induced systolic heart failure. (IF: 1.579)

Pasławska U, Kiczak L, Michlik K, Noszczyk-Nowak A, Pasławski R, Jonkisz P. Magazyn Weterynaryjny 2013, 22: 44-45. Niedotlenienie, czyli rola czynnika indukowanego hipoksją (HIF).

Skrzypczak P, Zyśko D, Pasławska U, Noszczyk-Nowak A, Janiszewski A, Kiczak L, Bania J, Zacharski M, Tomaszek A, Rybińska I, Nicpoń J, Pasławski R, Gajek J, Jankowska EA, Ponikowski P. Bull Vet Inst Pulawy, 2013, 57: 437–441. P-R interval in porcine model of chronic tachykardia-induced cardiomyopathy (IF: 0.365)

Pasławska U, Pasławski R, Janiszewski A, Noszczyk-Nowak A, Kiczak L, Zyśko D, Gajek J, Nicpoń J, Witkiewicz W, Jankowska EA, Szuba A, Ponikowski P. Journal of Animal and Veterinary Advances 2013, 12: 1260-1262. Normal M-mode Echocardiography of mitral valve in adult domestic swine.

Kołaczkowska A, Dyląg M, Kołaczkowski M.Biochem Biophys Res Commun. 2013 Mar 1;432(1):169-74. doi: 10.1016/j.bbrc.2013.01.047.Differential expression of the Candida glabrata CgRTA1 and CgRSB1 genes in response to various stress conditions (IF: 2.281).

Kolaczkowski M, Sroda-Pomianek K, Kolaczkowska A, Michalak K. Biochim Biophys Acta. 2013 Feb;1828(2):479-90. doi: 10.1016/j.bbamem.2012.10.024. A conserved interdomain communication pathway of pseudosymmetrically distributed residues affects substrate specificity of the fungal multidrug transporter Cdr1p (IF: 3.431)

2012

Pasławska U, Kurzok H, Kiczak L, Bania J. Med. Wet. 2012, 68: 349-352. Rola tlenku azotu w regulacji czynności układu krążenia. (IF: 0.203)

Kołaczkowska A, Manente M, Kołaczkowski M, Laba J, Ghislain M, Wawrzycka D. FEMS Yeast Res. 2012 May;12(3):279-92. doi: 10.1111/j.1567-1364.2011.00768.x. The regulatory inputs controlling pleiotropic drug resistance and hypoxic response in yeast converge at the promoter of the aminocholesterol resistance gene RTA1.

Kuźmińska-Bajor M., Kuczkowski M., Grzymajło K., Wojciech Ł., Sabat M., Kisiela D., Wieliczko A., Ugorski M. Vet. Microbiol. 2012 158: 205 – 210. Decreased colonization of chicks by Salmonella enterica serovar Gallinarum expressing mannose-sensitive FimH adhesin from Salmonella enterica serovar Enteritidis (IF: 3.127)

Kisiela D.I., Chattopadhyay S., Liby S.J., Karlinsey J.E., Fang F.C., Tchesnokova V., Kramer J.J., Beskhlebnaya V., Samadpour M., Grzymajło K., Ugorski M., Lankau E.W., Mackie R.I., Clegg S., Sokurenko E.V. PLoS Pathogens 2012 8: e1002733.2012). Evolution of Salmonella enterica virulence via point mutations in the fimbrial adhesin (IF: 8.136)

2011

Paslawska U, Gajek J, Kiczak L, Noszczyk-Nowak A, Skrzypczak P, Bania J, Tomaszek A, Zacharski M, Sambor I, Dziegiel P, Zysko D, Banasiak W, Jankowska EA, Ponikowski P. Int J Cardiol. 2011 Nov 17;153(1):36-41. doi: 10.1016/j.ijcard.2010.08.033. Development of porcine model of chronic tachycardia-induced cardiomyopathy (IF: 7.078)

Paslawska U, Gajek J, Kiczak L, Noszczyk-Nowak A, Skrzypczak P, Bania J, Tomaszek A, Zacharski M, Sambor I, Dziegiel P, Zysko D, Banasiak W, Jankowska EA, Ponikowski P. Int J Cardiol. 2011, 153: 36-41. Development of porcine model of chronic tachycardia-induced cardiomyopathy. (IF: 6.802)

Pula B., Jethon A., Piotrkowska A., Gomulkiewicz A., Owczarek T., Calik J., Wojna A., Witkiewicz W., Rys J., Ugorski M., Dzięgiel P., Podhorska Okolow M. (2011) Podoplanin expression by cancer-associated fibroblasts

Maj T, Switała-Jelen K, Miazek A, Szafarowicz-Basta B, Kiczak L, Slawek A, Chelmonska-Soyta A. Autoimmunity 2011, 44: 520-530. Effects of tamoxifen on estrogen receptor-α level in immune cells and humoral specific response after immunization of C3H/He male mice with syngeneic testicular germ cells (TGC).

Ugorski M., Kuźmińska-Bajor M., Kisiela D. (2011) Rola fimbrii typu 1 w patogenezie zakażeń pałeczkami Salmonella. Post. Mikrobiol. 50: 59 – 68. (IF: 0.145)

2010 & earlier

Dzięgiel P., Owczarek T., Plażuk E. Gomułkiewicz A., Majchrzak M., Podhorska Okołów M., Driouch K., Lidereau, Ugorski M. 2010 Ceramide galactosyltransferase (UGT8) is a molecular marker of breast cancer malignancy and metastases. Br. J. Cancer 103: 524 – 531. (IF: 4.396)

Grzymajło K., Kużmińska-Bajor M., Jaworski J., Dobryszycki P., Ugorski M. 2010 High-adhesive properties of FimH adhesin from Salmonella enterica serovar Enteritidis are determined by a single P118S substitution. Microbiology 156: 1738 – 1748. (IF: 3.11)

Noszczyk-Nowak A, Pasławska U, Janiszewski A, Kiczak L, Bania J, Tomaszek A, Zacharski M, Jankowska EA, Nicpoń J, Ugorski M, Ponikowski P, Banasiak W. Med Wet. 2010, 66: 378-383. Duże zwierzęta jako modele niewydolności serca. Rodzaje modeli, sposób wykonania i przydatność kliniczna.

Pasławska U, Dzięgiel P, Sambor I, Kiczak L, Pasławski R. Magazyn Weterynaryjny 2010, 19:1250-1252. Czy leczenie niewydolności serca może być proste?

Kolaczkowska A, Kolaczkowski M, Sokolowska A, Miecznikowska H, Kubiak A, Rolka K, Polanowski A. Mycoses. 2010 Jul;53(4):314-20. doi: 10.1111/j.1439-0507.2009.01722.x. The antifungal properties of chicken egg cystatin against Candida yeast isolates showing different levels of azole resistance. (IF: 1.53)

Kolaczkowski M, Kolaczkowska A, Sroda K, Ramalhete C, Michalak K, Mulhovo S, Ferreira MJ. Mycoses. 2010 Jul;53(4):305-10. doi: 10.1111/j.1439-0507.2009.01711.x.Substrates and modulators of the multidrug transporter Cdr1p of Candida albicans in antifungal extracts of medicinal plants. (IF: 1.53)

Pasławska U, Dzięgiel P, Sambor I, Kiczak L, Pasławski R. Weter Prakt. 2010, 7: s.34, 36-37. Przewlekła niewydolność serca. Czy zwłóknienie ma znaczenie?

Król M., Polańska J., Pawłowski K.M., Turowski P., Skierski J., Majewska A., Ugorski M., Morty R.E., Motyl T. J. Appl. Genet. 2010 51: 37 – 50 Transcriptomic signature of cell lines isolated from canine mammary adenocarcinoma metastasis to lungs.

Pawlas-Opiela M., Wojcjech Ł., Sołtysiak Z., Otranto D., Ugorski M. Vet. Parasitol. 2010 169: 219 – 221, doi. 10.1016/j.vetpar.2009.12.030. Molecular comparison of Gasterophilus intestinalis and Gasterophilus nasalis from two distinct areas of Poland and Italy based on cox1 sequence analysis.

Grzymajło K., Kużmińska-Bajor M., Jaworski J., Dobryszycki P., Ugorski M. Microbiology 2010 156: 1738 – 1748; doi.10.1099/mic.0.039206-0. High-adhesive properties of FimH adhesin from Salmonella enterica serovar Enteritidis are determined by a single P118S substitution.

Król M., Pawłowski K.M., Skierski J., Turowski P., Majewska A., Polańska J., Ugorski M., Morty R.E., Motyl T. J. Appl. Genet. 2010 51: 169 – 183. Transcriptomic „portraits” of canine mammary cancer cell lines with different phenotype.

Dzięgiel P., Owczarek T., Plażuk E. Gomułkiewicz A., Majchrzak M., Podhorska-Okołów M., Driouch K., Lidereau, Ugorski M. Br. J. Cancer 2010 103: 524 – 531.Ceramide galactosyltransferase (UGT8) is a molecular marker of breast cancer malignancy and metastases.

Kolaczkowski M, Kolaczkowska A, Motohashi N, Michalak K. Antimicrob Agents Chemother. 2009 Apr;53(4):1516-27. doi: 10.1128/AAC.00956-08.New high-throughput screening assay to reveal similarities and differences in inhibitory sensitivities of multidrug ATP-binding cassette transporters.

Kolaczkowski M, Kolaczkowska A, Stermitz FR. Microb Drug Resist. 2009 Mar;15(1):11-7. doi: 10.1089/mdr.2009.0854. Modulation of the antifungal activity of new medicinal plant extracts active on Candida glabrata by the major transporters and regulators of the pleiotropic drug-resistance network in Saccharomyces cerevisiae.

Nowak M., Madej J., Dziegiel P., Łopuszyński W., Rodo A., Ugorski M. Vet. Pathol. 2009 46: 222 – 226. Tumor-associated carbohydrate antigens: sialyl Lea and T/Tn antigens in canine mammary tumors.

Wałecka E., Bania J., Dworniczak E., Ugorski M. Lett. Appl. Microbiol. 2009 79 – 84.Genotypic characterization of hospital Enterococcus faecalis strains using MLVA.

Platt-Samoraj A., Szweda W., Ugorski M. Pol. J. Vet. Sciences 2009 12: 189 - 193. Isolation of Yersinia enterocolitica from aborted fetuses and sows in pig farms with reproductive disturbances.

Kiczak L., Pasławska U., Bania J., Ugorski M., Sambor I., Kochman A., Blach J., Chełmońska-Soyta A. Cytokine 2008 44: 352-360 (doi: 10.1016./j.cyto.2008.10.002).Increased expression of interleukin-1β and its novel splice variant in canine hearts with volume overload.

Kiczak L, Paslawska U, Bania J, Ugorski M, Sambor I, Kochman A, Blach J, Chelmonska-Soyta A. Cytokine 2008, 44: 352–360. Increased expression of interleukin-1b and its novel splice variant in canine hearts with volume overload.

Pasławska U., Kiczak L., Ugorski M., Chełmońska-Soyta A. Med. Wet 2008 64: 969-972. Wpływa cytokin propozaplanych (TNFa, Il-1 i Il-6) na czynność serca.

Kisiela D., Laskowska A., Sapeta A., Kuczkowski M., Wieliczko A., Ugorski M. 2006 Functional characterization of the FimH adhesin from Salmonella enterica serovars Enteritidis. Microbiology 152: 1337 – 1346.

Kisiela D., Laskowska A., Sapeta A., Kuczkowski M., Wieliczko A., Ugorski M. Microbiology 2006 152: 1337 – 1346.Functional characterization of the FimH adhesin from Salmonella enterica serovars Enteritidis.

Platt-Samoraj A., Ugorski M., Szweda W., Szczerba-Turek A., Wojciech Ł., Procajło Z. J. Vet. Med. 2006 B 341 – 346. Analysis of the presence of ail, ystA, and Yst B genes in Yersinia enterocolitica strains isolated from aborting sows and aborted fetuses.

Wojciech Ł., Bednarski M., Wieliczko A., Ugorski M. Pol. J. Vet. Sciences 2005 8: 41 - 47. Genotype analysis of Campylobacter jejuni isolated from broilers in Poland.

Kisiela D., Sapeta A., Kuczkowski M., Stefaniak T., Wieliczko A., Ugorski M. Infect. Immun. 2005 73: 6187 – 6190. Characterization of FimH adhesins expressed by Salmonella enterica serovar Gallinarum biovars Gallinarum and Pullorum: reconstitution of mannose-binding properties by single amino acid substitution.

Kisiela D., Kuczkowski M., Kiczak L., Wieliczko A., Ugorski M. J. Vet. Med. 2005 B 52: 214 – 218. Differentiation of Salmonella galliarum biovar Gallinarum from Salmonella gallinarum biovar Pullorum by PCR-RFLP of the fimH gene.

Kisiela D., Kiczak L., Kuźmińska M., Kuczkowski M., Franiczak R., Ugorski M. Med. Wet. 2005 61: 1259 – 1262. Analiza genu fimH kodującego adhezynę fimbrii typu 1 S. enterica serovar Enteritidis.

Kisiela D, Kuczkowski M, Kiczak L, Wieliczko A, Ugorski M. J Vet Med B Infect Dis Vet Public Health. 2005, 52: 214-218. Differentiation of Salmonella Gallinarum biovar Gallinarum from Salmonella Gallinarum biovar Pullorum by PCR-RFLP of the fimH gene.

Kisiela D, Kiczak L, Kuźmińska M, Kuczkowski M, Franiczek R, Ugorski M. Med. Wet. 2005, 61: 1259-1262. Analiza genu fimH kodującego adhezynę fimbrii typu 1 Salmonella enterica serowar Enteritidis.

Kisiela D., Sapeta A., Kuczkowski M., Stefaniak T., Wieliczko A., Ugorski M. 2005 Characterization of FimH adhesins expressed by Salmonella enterica serovar Gallinarum biovars Gallinarum and Pullorum: reconstitution of mannose-binding properties by single amino acid substitution. Infect. Immun. 73: 6187 – 6190.

Kisiela D., Kuczkowski M., Kiczak L., Wieliczko A., Ugorski M. 2005 Differentiation of Salmonella galliarum biovar Gallinarum from Salmonella gallinarum biovar Pullorum by PCR-RFLP of the fimH gene. J. Vet. Med. B 52: 214 – 218.      

Kisiela D., Kiczak L., Kuźmińska M., Kuczkowski M., Franiczak R., Ugorski M. 2005 Analiza genu fimH kodującego adhezynę fimbrii typu 1 S. enterica serovar Enteritidis. Med. Wet. 61: 1259 - 1262.

Wojciech Ł., Staroniewicz Z., Jakubczak A., Ugorski M. J. Vet. Med. B 2004 51: 238 – 244. Typing of Yersinia enterocolitica isolates by ITS profiling, REP- and ERIC-PCR.

Wojciech Ł., Kowalczyk K., Staroniewicz Z., Kosek K., Molenda J., Ugorski M. Bull. Vet. Inst. Puławy 2004 48: 427 – 435. Genotypic characterization of Listeria monocytogenes isolates from foodstuffs and animals in Poland.

Kuczkowski M., Wieliczko A., Kisiela D., Mazurkiewicz M., Ugorski M. Bull. Vet. Inst. Puławy 2004 8: 375 - 382. Immunisation of hens with Salmonella Enteritidis recombinant fimbrial proteins SefA, FimH and AgfA. Analysis of cellular response and protective effect against Salmonella Enteritidis infection.

Research equipment

  • NightOWL LB 983 in vivo tumor growth and microbial infection imaging system (Berthold Technologies),
  • 96-well plate reader/spectrophotometer Multiscan EX with, 405, 414, 450, 492, 540, 570, 620, 690 nm filters (Thermo),
  • Ascent FL Fluorimeter/Luminometer with Ex: 485, 355, 390 nm, Em: 460, 538 nm filters, heating, compatible with 6-, 24- i 96-well plates with top and bottom reading (Thermo),
  • Inverted Fluorescent Microscope Eclipse TE2000-S with camera, 4x, 10x, 20x i 40x objectives, and GA-2 EX510-566nm/DM575nm/BA590nm, B2-A EX450-490nm/DM505nm/BA520nm filters (Nikon),
  • Inverted MicroscopeAE-20 (Motic),
  • UV-VIS Spectrophotometers U-5100 (Hitachi), SmartSpec 3000 (Biorad),
  • Avanti J-20XPI high speed, high capacity centrifuge w/cooling, with JA-10 (6x 500ml, 17000xg) rotor (Beckman Coulter),
  • Gene Pulser Xcell electroporator with PC & CE modules (Biorad),
  • Savant SpeedVac SC110A Vacum concentrator with refrigerated vapor trap RVT400 (Thermo),
  • Innova U535 deepfreezer (New Brunswick Scientific),
  • D-78532 cooled centrifuge w/rotor set (Hettich),
  • 3K18 centrifuge (Sigma),
  • Titramax 1000 heated 96-well plates shaker (Heildorph),
  • HT Minitron microbiological incubation shaker (Infors),
  • Forma Class II A2 Biosafety Cabinet (Thermo),
  • Cell culture CO2 incubators Nuaire i Thermo,
  • Stericlave 15S & 24B autoclaves (Cominox),
  • RE120 Coolled waterbath RE120 (LAUDA),
  • Sono Plus Hd2070 sonifier (Bandelin),
  • PRO 200 rotor-stator homogenizer for 2 ml tubes,
  • DNA agarose gel electrophoresis set (Bio-Rad, Biometra),
  • SDS-PAGE and Western Blotting set (Bio-Rad),
  • Proflex 3x32 wells Thermocycler (Applied Bioscience) and T3 Thermocycler (Biometra),
  • Small laboratory equipement

Log in