Division of Histology and Embryology

Division of Histology and Embryology

ul. Norwida 25
50-375 Wrocław

phone 71 320 5464 fax 71 320 5465

Head of Division
dr Piotr Kuropka, adiunkt, phone 71 320 5487 email This email address is being protected from spambots. You need JavaScript enabled to view it.

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Division's staff
dr Violetta Kapuśniak, adiunkt phone 71 320 5462 email This email address is being protected from spambots. You need JavaScript enabled to view it.
dr Renata Nowaczyk, adiunkt phone 71 320 5465 email This email address is being protected from spambots. You need JavaScript enabled to view it.
dr hab. Jan Madej, prof. UPWr phone 71 320 5486 email This email address is being protected from spambots. You need JavaScript enabled to view it.
dr Katarzyna Kaleta-Kuratewicz, specjalista phone 71 320 5463
email This email address is being protected from spambots. You need JavaScript enabled to view it.
mgr Elżbieta Krasowska, st. technik phone 71 320 5238 email This email address is being protected from spambots. You need JavaScript enabled to view it.

Professor emeritus
prof. dr hab. Jan Kuryszko, profesor zwyczajny email This email address is being protected from spambots. You need JavaScript enabled to view it.

PhD student
lek. wet. Joanna Skonieczna


The Department conducts research in cooperation with other scientific centers in the field of histophysiology of organs and tissues. They concern mainly hard tissues such as bone and cartilage tissue and tooth tissues in various experimental systems of both contemporary and fossil material. In addition, research which investigate various aspects of changes in organs, both in the biological and in the veterinary sciences. Histological and smear specimens in routine staining and histochemical and immunohistochemical staining are performed. In addition, for a limited time, live cells derived from cell cultures and their response to various factors modulating their activity (mitochondria and cytoskeleton) are tested. The analyzes are performed using light and fluorescence microscopy along with a variety of morphometric tests assisted by Nis Elements AR software.

The effects of prebiotics and synbiotics on the histological structures of lymphatic organs in broilers. Team: Jan Madej, Marek Bednarczyk, Tadeusz Stefaniak
The condition of the immune system in chicken plays a significant role in the contemporary intensive poultry production. The ability of the immune system to respond to the environmental antigens, as well as efficacy of the immunisation, depends mainly on the level of development and condition of the system. Previous studies indicate that the type of intestinal microorganisms (microbiome) can influence the growth and health of their host (Fooks et al. 2002, Bednarczyk et al. 2011). Prebiotics and synbiotics (prebiotic + probiotic) influence the intestinal microbiome of the bird, can stimulate the gut-associated lymphoid tissue (GALT) and modulate the immune response of the host (Cesta 2006, Haghighi et al. 2006, 2008, Brisbin et al. 2011).
The study aimed to investigate the effects of in ovo-injected prebiotics and synbiotics on the histological structures and cell composition of lymphatic organs in broilers. Prebiotics and synbiotics, were injected into the egg: control group (C) –physiological saline; prebiotic 1 (Pre1) – inulin; prebiotic 2 (Pre2) – commercially developed, nondigestible transgalacto-oligosaccharide Bi2tos; synbiotic 1 (Syn1) – inulin + Lactococcus lactis subsp. lactis or synbiotic 2 (Syn2) – Bi2tos + L. lactis subsp. cremoris. The material for the study consist of thymus, bursa of Fabricius, spleen, distal end of ileum, and cecal tonsil taken from chickens on day 1, 7, 21, and 35 after hatching.
The results of this study indicate the immunomodulatory effect of pre- and synbiotics on the structure and cell composition of central and peripheral lymphatic organs of the chickens. Both the bioactives injected in ovo at day 12 of incubation had no adverse effect on the development of immune system in chickens. At day 7, after hatching, the decrease in B-cell number in BF was observed (Pre1, Pre2, and Syn2), as well as more pronounced colonisation of CT by T cells in all experimental groups and by B cells in both synbiotic-treated groups was also found. On day 21 Syn2 decreased the cortex/medulla ratio in the thymus, slowed the development of the cortex in bursal follicles and stimulated the B-dependent area in the spleen. At the same time both the synbiotics exerted more potent stimulatory effect on GALT colonisation by T cells than prebiotics alone. Similar effect in relation to B-cell number between the Syn2 and Pre2 groups was found.
(This research was carried out in cooperation with Department of Animal Biochemistry and Biotechnology, UTP University of Science and Technology, Bydgoszcz)


Dzimira S., Kapuśniak V., Madej J.A. Immunohistochemical diagnostic of hibernoma in dog. Pol. J. Vet. Sci. 2015, 1:233–236. (IF: 0.719)

Gasior-Glogowska M., Komorowska M., Hanuza Jerzy., Maczka M., Zajac A. Ptak M., Bedzinski R., Kobielarz M., Maksymowicz K., Kuropka P., Szotek S. FT-Raman spectroscopic study of human skin subjected to uniaxial stress. J. Mech. Behav. Biomed. Mater. 2013, 18:240-252 (IF: 2.313)

Kasztura M., Sniezewski L., Laszkiewicz A., Majkowski M., Kobak K., Peczek K., Janik S., Kapusniak V., Miazek A., Cebrat M., Kisielow P. Search for the function of NWC, third gene within RAG locus: generation and characterization of NWC-deficient mice. Arch. Immunol. Ther. Exp. (Warsz). 2016, 64(4):311–319. (IF 2.464)

Kiczak L., Tomaszek A., Paslawska U, Bania J., Noszczyk-Nowak A., Skrzypczak P., Paslawski R., Zacharski M., Kuropka P., Janiszewski A., Ponikowski P., Jankowska E.A. Sex differences in porcine left ventricular myocardial remodeling due to right ventricular pacing. Biol. Sex Differ. 2015, 6:32. (IF: 3.237)

Kiełbowicz Z., Piatek A., Biezynski J., Skrzypczak P., Kuropka P., Kuryszko J., Nikodem A., Kafarski P., Pezowicz C. The experimental osteoporosis in sheep - clinical approach Pol. J. Vet. Sci. 2015, 18(3):645–54. (IF: 0.719)

Kiełbowicz Z., Piątek A., Kuropka P., Mytnik E., Nikodem A., Bieżyński J., Skrzypczak P., Pezowicz C., Kuryszko J., Reichert P. Experimental osteoporosis in sheep – mechanical and histological approach. Pol. J. Vet. Sci. 2016, 19(1):109–118. (IF: 0.719)

Kiełbowicz Z., Ploneczka-Janeczko K., Kuropka P. Insight into behavior of epithelial cells of the feline conjunctiva in chronic conjunctivitis as a possible limitation in detection of Chlamydophila spp. Pol. J. Vet. Sci. 2014, 17(3):441-445. (IF: 0.604).

Kobielarz M., Szotek S., Kuropka P., Maksymowicz K., Morawska-Soltysik M. Mechanical properties of the cerebrum: application in neurosurgical procedures. Strain 2011, 47(4):382-387 (IF: 1.103)

Kosiorowska A., Puggaard L., Hedemann M.S., Sehested J., Jensen S.K., Kristensen N.B., Kuropka, P., Marycz K., Vestergaard M. Gastrointestinal development of dairy calves fed low- or high-starch concentrate at two milk allowances. Animal 2011, 5(2):211-219. (IF: 1.744)

Madej J.P., Bednarczyk M. Effect of in ovo-delivered prebiotics and synbiotics on the morphology and specific immune cell composition in the gut-associated lymphoid tissue. Poultry Sci. 2016, 95:19–29. (IF: 1.685)

Madej J.P., Nowaczyk R.M., Janeczek M., Chrószcz A., Korczyński M. The effect of dietary supplementation with chromium-enriched soya meal on lymphatic cells in caecal tonsil of laying hens. Anim. Feed Sci. Tech. 2017, 223: 53-58. (IF 1.713).

Madej J.P., Stefaniak T., Bednarczyk M. Effect of in ovo-delivered prebiotics and synbiotics on lymphoid-organs’ morphology in chickens. Poultry Sci. 2015, 94:1209–1219. (IF: 1.685)

Madej J.P., Woźniakowski G., Gaweł A. Morphology of immune organs after very virulent plus strain of Marek’s disease virus infection in vaccinated hens. Pol. J. Vet. Sci. 2016, 19(2):325–335. (IF 0.719).

Maj T., Sławek A., Kaleta-Kuratewicz K., Chełmońska-Soyta A. 17ß-Estradiol and interferon tau interact in the regulation of the immune response in a model of experimental autoimmune orchitis. J Interferon Cytokine Res. 2011, 31(11):825-837. (IF: 3.063)

Miśta D., Króliczewska B., Pecka-Kiełb E., Kapuśniak V., Zawadzki W., Graczyk S., Kowalczyk A., Łukaszewicz E., Bednarczyk M. Effect of in ovo injected prebiotics and synbiotics on the caecal fermentation and intestinal morphology of broiler chickens. Anim. Prod. Sci. published online: 29 August 2016. (IF 0.902)

Reichert P., Kiełbowicz Z., Dzięgiel P., Puła B., Kuryszko J., Gosk J., Bocheńska A. The rabbit brachial plexus as a model for nerve repair surgery – histomorphometric analysis. Anat Rec (Hoboken). 2015, 298(2):444-454. (IF: 1.507)

Reichert P., Kiełbowicz Z., Dzięgiel P., Puła B., Kuryszko J., Wrzosek M., Kiełbowicz M., Gosk J. Collateral sprouting axons of end-to-side nerve coaptation in the avulsion of ventral branches of the C5-C6 spinal nerves in the brachial plexus. Folia Neuropathol. 2015, 53(4):327–342. (IF: 1.233)

Reichert P., Kiełbowicz Z., Kuryszko J., Bocheńska A., Puła B., Rutowski R. Side to side coaptation--new technic in peripherial nerve surgery--preliminary report. Pol. J. Vet. Sci. 2016, 19(1):213–215. (IF: 0.719)

Szydłowski P., Madej J.P., Mazurkiewicz-Kania M. Ultrastructure and distribution of chromatophores in the skin of the leopard gecko (Eublepharis macularius). Acta Zoologica (Stockholm), 2016, 97: 370–375. (IF: 0.989)

Tomaszek A., Kiczak L., Bania J., Paslawska U., Zacharski M., Janiszewski A., Noszczyk-Nowak A., Dziegiel P., Kuropka P., Ponikowski P., Jankowska E.A. Increased gene expression of catecholamine-synthesizing enzymes in adrenal glands contributes to high circulating catecholamines in pigs with tachycardia-induced cardiomyopathy. J. Physiol. Pharmacol. 2015, 66(2):227-231. (IF: 2.804)

Zacharski M., Orlowska A., Kiczak L., Kuropka P., Pawlak A., Paslawska U. Studies on the alternative IL-transcript expression in peripheral blood leukocytes in chronic heart failure in dogs. J. Comp. Pathol. 2016, 154(1):82 (IF: 1.173)

Zimecki M., Artym J., Kocięba M., Kaleta-Kuratewicz K., Kuropka P., Kuryszko J., Kruzel M. Homologous Lactoferrin Triggers Mobilization of the Myelocytic Lineage of Bone Marrow in Experimental Mice. Stem Cells Dev. 2013, 22(24):3261–3270. (IF: 4.202)

Zimecki M., Artym J., Kocięba M., Kaleta-Kuratewicz K., Kruzel M.L. Lactoferrin restrains alergen-induced pleurisy in mice. Inflamm. Res. 2012, 61(11):1247-1255. (IF: 1.964)

Scientific monographs

Kuryszko J., Madej J.P., Kapuśniak V. Guidebook to animals histology. UWP Wrocław 2012 (second edition, extended).

PhD theses

Author, year, director of the thesis
• Agnieszka Dziewicka, PhD, 2012, Title: Bone morphogenetic proteins as chondrocyte stabilizing factors for autograft use by autocrine regulation. Director of the thesis Jan Kuryszko
• Maciej Kiełbowicz, PhD, 2014, Title: Influence of antlerogenic stem cells and their homogenate on anterior corneal epithelium and stromal wound healing in rabbits. Director of the thesis Jan Kuryszko
• Jakub Grzesiak, PhD, 2015, Title: Characterizations and use of autologic mezenchymal stem cells derived from adipose tissue (ASC) of dogs. Director of the thesis Jan Kuryszko
• Dorota Wirska, PhD, 2016, Title: Induction and development of tooth in dogs. Director of the thesis Jan Kuryszko

Research equipment

• Fluorescence microscope Eclipse 80i (Nikon) with differential interference kontrast (DIC, Nomarski) with a video camera.
• Tissue processor Citadel 1000 (Shandon)
• Embedding Center Histocentre 2 (Shandon)
• Microtome HM310 (Microm)
• Flattening table HI 1220 (Leica)
• pH Meter HI 221 (Hanna)
• Water bath ML 547 (AJL Electronics)
• Laboratory incubators (thermostats) (Incucell i Advantage-Lab)
• Water demineralization system HLP Smart 2000S (Hydrolab)

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